GFP anticorps (CypHer5E)

N° du produit ABIN1742297

L’anticorps Lapin Polyclonal anti-GFP a été validé pour ICC. Il convient pour détecter GFP dans des échantillons de Aequorea victoria. Il y a 1 publication disponible.

Aperçu rapide pour GFP anticorps (CypHer5E) (ABIN1742297)

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GFP est conjugé à/à la CypHer5E

Application: Immunocytochemistry (ICC)

Détail du produit anti-GFP anticorps (CypHer5E)

Réactivité croisée (Details): Cross reacts with all GFP derivatives tested so far (e.g. EYFP, ECFP, EGFP).

Purification: Affinity purified with the immunogen, fluorescence-labeled with CypHer5E. Rabbit serum albumin was added for stabilization.

Immunogène: Strep-Tag® fusion protein, full-length GFP.

Information d'application

Indications d'application: IP: not tested yet
ICC: 1 : 100 up to 1 : 1000
IHC-P: not tested yet

Commentaires:

ABIN1742297 Suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20 °C until use, or store aliquots at -80 °C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.

Buffer: PBS

Conseil sur la manipulation: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Stock: -20 °C

Stockage commentaire: Fluorescence-labeled antibodies should be reconstituted immediately upon delivery.
Prolonged storage at 4 °C is not recommended!

Références pour anticorps anti-GFP (CypHer5E) (ABIN1742297)

Tatavarty, Sun, Turrigiano: "How to scale down postsynaptic strength." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 32, pp. 13179-89, (2013) (PubMed).

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: GFP